Development of a high-efficiency gene knockout system for Pochonia chlamydosporia
نویسندگان
چکیده
منابع مشابه
A novel method for promoting conidial production by a nematophagous fungus, Pochonia chlamydosporia AS6.8
A novel method was described for promoting conidial production of nematophagous fungus, Pochonia chlamydosporia AS6.8, by hydrogen peroxide (H2O2) treatment and two-stage cultivation. The fungi were first grown on potato dextrose agar plates to encourage vegetative growth, after briefly treating with 90 mM concentrations of H2O2, and then were transferred to water agar (WA) plates for sporulati...
متن کاملSequencing and functional analysis of the genome of a nematode egg-parasitic fungus, Pochonia chlamydosporia.
Pochonia chlamydosporia is a worldwide-distributed soil fungus with a great capacity to infect and destroy the eggs and kill females of plant-parasitic nematodes. Additionally, it has the ability to colonize endophytically roots of economically-important crop plants, thereby promoting their growth and eliciting plant defenses. This multitrophic behavior makes P. chlamydosporia a potentially use...
متن کاملAppendix 1 Methodology for studies on Pochonia chlamydosporia isolates (Rothamsted Research)
Cultures of P. chlamydosporia from infected nematode eggs in soil from different parts of the world have been freeze-dried and retained at Rothamsted Research, prior to use. Eight isolates (five collected from cyst nematode eggs and three from root-knot nematode eggs) were grown on 1.7% corn meal agar (Oxoid, Basingstoke, UK) at 25C prior to use. Chlamydospores used as inoculum were produced in...
متن کاملBiological control of root-knot nematodes (Meloidogyne spp.) by the fungus Pochonia chlamydosporia
5 Introduction 6 Introduction to root-knot nematodes (Meloidogyne) 6 The life cycle of Meloidogyne species 8 Integrated pest management and biological control 11 Nematophagous fungi 12 Pochonia chlamydosporia (syn. Verticillium chlamydosporium) 12 Materials and Methods 14 Results and Discussion 15 P. chlamydosporia as a control agent of root knot nematodes 15 Conclusions 21 References 23
متن کاملThe Pochonia chlamydosporia Serine Protease Gene vcp1 Is Subject to Regulation by Carbon, Nitrogen and pH: Implications for Nematode Biocontrol
The alkaline serine protease VCP1 of the fungus Pochonia chlamydosporia belongs to a family of subtilisin-like enzymes that are involved in infection of nematode and insect hosts. It is involved early in the infection process, removing the outer proteinaceous vitelline membrane of nematode eggs. Little is known about the regulation of this gene, even though an understanding of how nutrients and...
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ژورنال
عنوان ژورنال: Microbiological Research
سال: 2015
ISSN: 0944-5013
DOI: 10.1016/j.micres.2014.10.001